Radioassay of serum folate.
نویسندگان
چکیده
We describe a radioassay procedure for serum folate, in which commercially available crystalline 3lactoglobulirt is used as the folate-binding agent. The folate binders present in serum are eliminated by heating serum in a boiling water bath for 15 mm to denature the proteins. Radioassayable serum folate is shown to be stable under these conditions, and the results for folate compare well with those obtained by a microbiological assay.
منابع مشابه
Clinical and laboratory observations on serum folate-binding protein.
We studied the effect of serum folate-binding protein (FBP) on folate radioassays and the relationship of the serum level of unsaturated FBP to the serum folate level in various clinical states. Our modification of a heat-extracted radioassay was compared to a whole serum radioassay. Our results confirmed the existence of elevated serum levels of unsaturated FBP in some normal subjects, in some...
متن کاملAnalytical evaluation of a folate radioassay: application to serum and erythrocyte measurements.
Many commercial folate radioassay kits have proven to be unsatisfactory in the past. It has been found that the Schwarz/Mann Iodine-125 folate radioassay kit permits a rapid measurement of folic acid requiring only a single incubation at pH 9.3. At this pH, the relative affinity of pteroylglutamic acid (FA) and N-5-methyltetrahydrofolic acid (MTFA) for the milk binder is the same. Additionally,...
متن کاملRadioassay of serum folate, with use of a serum blank and nondialyzed milk as folate binder.
Serum folate determination by this method requires incubation of 0.1 ml of serum (or standards) with nondialyzed milk and then with tritium-labeled folate. A serum blank corrects for nonspecific binders present in the serum. Unbound folate is removed with hemoglobin-coated charcoal. The standard deviation of the results is ±G.2 pig/liter, and more than 90% of folate added to the serum is accoun...
متن کاملResults with commercial radioassay kits compared with microbiological assay of folate in serum and whole-blood.
We compared results with three commercial folate radioassay kits [Bio-Rad, New England Nuclear (NEN), and RIA Products] with those by microbiological assay for more than 200 samples of human serum and whole blood. All but one kit (NEN) compared favorably with the microbiological assay for serum samples, although there were notable diagnostic discrepancies. Two kits (NEN and Bio-Rad) were tested...
متن کاملComparison of radioassay and microbiological assay for serum folate, with clinical assessment of discrepant results.
Folate assays by use of radiolabeled folate provide obvious practical advantages over the standard microbiological assay, but remain incompletely tested. We therefore compared results for 415 sera with a kit involving 3H-labeled folate and the Lactobacillus casei microbiological method. We examined the patients' data when there were discrepancies between the two methods. Although the correlatio...
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ورودعنوان ژورنال:
- Clinical chemistry
دوره 19 10 شماره
صفحات -
تاریخ انتشار 1973